Contents
* Isolation of DNA fragments using glass milk (GENE-CLEAN)
* SOUTHERN BLOT
* SOUTHERN ANALYSIS OF MOUSE TOE/TAIL DNA
* Northern transfer protocol
* Hybridizing Northern blots with riboprobes
* cDNA + GENOMIC DNA LIBRARY SCREENING
* Isolation of Mouse genomic DNA
* RNA ISOLATION USING GUHCL
* POLY A+ RNA PREPARATION using proteinase K and SDS
* SELECTION OF POLYADENYLATED RNA FROM TOTAL RNA
* Mini plasmid preps
* Large Scale Plasmid Preps
* Preparing and USING bacterial cells for electroporation
* In situ hybridisation to RNA in tissue sections
* PRIMER LABELLING FOR PRIMER EXTENSION ASSAYand PRIMER EXTENSION
* DNA-se I FOOTPRINTING
* Double stranded DNA sequencing (Dideoxy)
* Buffer gradient SEQUENCING GELS
* Nested Deletions using exonuclease-III and mung bean nuclease
* SITE DIRECTED MUTAGENESIS USING THE UNG - DUT SYSTEM.
* In vitro mutagenesis using Altered Sites
* Labeling of oligonucleotides
o RANDOM HEXAMER LABELLING OF CDNA PROBES
o RNAse protection assay
o Isolation of phage DNA
o PHAGE PURIFICATION WITH DE52
o BUTANOL PRECIPITATION OF DEPROTECTED OLIGONUCLEOTIDES
o OLIGO PURIFICATION on acrylamide gels
o POLYMERASE CHAIN REACTION
o Rapid Amplification of mRNA ENDS by PCR (RACE)
o YEAST MEDIA
o TRANSFORMATION OF YEAST
o Modified yeast transformation
o Isolation of shuttle vectors from yeast and transformation into E. coli
o Yeast lysates for westerns
澳大利亚墨尔本大学的分子生物学实验室实验手册，包括了分子生物学实验室常见实验操作。
