将原始序列或FASTA格式的序列拷贝到以下区域中 >sample sequence ctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgagcgcgcgtaatacgactcactatagggcgaattggagctccaccgcggtggcggccgctctagaactagtggatcccccgggctgcaggaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccagcttttgttccctttagtgagggttaattgcgcgcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccac
以下输入限制位点公式 ，缺省的公式为常用限制位点公式。斜线标出匹配的限制位点，圆括号标出位点名称随后的数字为从识别位点 3'端算起断裂的位置。在位点序列中方括号表示为同一个位置可能的碱基。每个公式后以逗号分隔，最后一个公式不带逗号。识别非回文序列位点的酶输入两条公式，每个公式代表一个识别序列。 /gacgtc/ (AatII gacgt|c)1, /tccgga/ (AccIII t|ccgga)5, /agct/ (AluI ag|ct)2, /gggccc/ (ApaI gggcc|c)1, /c[ct]cg[ag]g/ (AvaI c|ycgrg)5, /ggatcc/ (BamHI g|gatcc)5, /tgatca/ (BclI t|gatca)5, /agatct/ (BglII a|gatct)5, /gcgcgc/ (BssHII g|cgcgc)5, /atcgat/ (ClaI at|cgat)4, /tttaaa/ (DraI ttt|aaa)3, /gaattc/ (EcoRI g|aattc)5, /gatatc/ (EcoRV gat|atc)3, /gt[ct][ag]ac/ (HincII gty|rac)3, /aagctt/ (HindIII a|agctt)5, /ga[gatc]tc/ (HinfI g|antc)4, /gttaac/ (HpaI gtt|aac)3, /ccgg/ (HpaII c|cgg)3, /ggtacc/ (KpnI ggtac|c)1, /[gatc]gatc[gatc]/ (MboI |gatc)5, /gaaga[gatc][gatc][gatc][gatc][gatc][gatc][gatc][gatc]/ (MboII gaagannnnnnnn|)0, /[gatc][gatc][gatc][gatc][gatc][gatc][gatc][gatc]tcttc/ (MboII n|nnnnnnntcttc)12, /acgcgt/ (MluI a|cgcgt)5, /ccgg/ (MspI c|cgg)3, /gccggc/ (NaeI gcc|ggc)3, /ggcgcc/ (NarI gg|cgcc)4, /ccatgg/ (NcoI c|catgg)5, /catatg/ (NdeI ca|tatg)4, /gatc/ (NdeII |gatc)4, /gctagc/ (NheI g|ctagc)5, /gcggccgc/ (NotI gc|ggccgc)6, /tcgcga/ (NruI tcg|cga)3, /ctgcag/ (PstI ctgca|g)1, /cgatcg/ (PvuI cgat|cg)2, /cagctg/ (PvuII cag|ctg)3, /gtac/ (RsaI gt|ac)2, /gagctc/ (SacI gagct|c)1, /ccgcgg/ (SacII ccgc|gg)2, /gtcgac/ (SalI g|tcgac)5, /agtact/ (ScaI agt|act)3, /cccggg/ (SmaI ccc|ggg)3, /actagt/ (SpeI a|ctagt)5, /gcatgc/ (SphI gcatg|c)1, /aatatt/ (SspI aat|att)3, /aggcct/ (StuI agg|cct)3, /tcga/ (TaqI t|cga)3, /attaat/ (VspI at|taat)4, /tctaga/ (XbaI t|ctaga)5, /ctcgag/ (XhoI c|tcgag)5, /cccggg/ (XmaI c|ccggg)5