将原始序列或FASTA格式的序列拷贝到以下区域中 >mRNA /gene="fem-2" (exons in uppercase) gaacgcgaatgcctctctctctttcgatgggtatgccaattgtccacattcactcgtgttgcctcctctttgccaacacgcaagacaccagaaacgcgtcaaccaaagagaaaaagacgccgacaacgggcagcactcgcgagagacaaaggttatcgcgttgtgttattatacattcgcatccgggtcaactttagtccgttgaacatgcttcttgaaaacctagttctcttaaaataacgttttagaagttttggtcttcagATGTCTGATTCGCTAAATCATCCATCGAGTTCTACGGTGCATGCAGATGATGGATTCGAGCCACCAACATCTCCGGAAGACAACAACAAAAAACCGTCTTTAGAACAAATTAAACAGGAAAGAGAAGCGTTGTTTACGgttagttacctattagctgcaagttttgaaaaagcggaatctgtaaaaagcggaatctgtaaaaaaaacatctaaggaataattctgaaaagaaaaagtttctaaatgttaatcggaatccaatttttatgaaattatttaaaaaaaaactaaaattagtttctaaaaaatttttctaaagtaattggaccatgtgaaggtacacccacttgttccaatatgccatatctaactgtaaaataatttgattctcatgagaatatttttcagGATCTATTCGCAGATCGTCGACGAAGCGCTCGTTCTGTGATTGAAGAAGCTTTCCAAAACGAACTCATGAGTGCTGAACCAGTCCAGCCAAACGTGCCGAATCCACATTgtgagttggaaatttttatttgataaccaagagaaaaaaagttctacctttttttcaaaaacctttccaaaaatgattccatctgatataggattaagaaaaatattttccgaaatctctgcttttcagCGATTCCCATTCGTTTCCGTCATCAACCAGTTGCTGGACCTGCTCATGATGTTTTCGGAGACGCGGTGCATTCAATTTTTCAAAAAATAATGTCCAGgtatacactatttttgcatatttttcttgccaaatttggtcaaaaaccgtagtacaacccaaaaagtttcttcatttcagAGGAGTGAACGCGGATTATAGTCATTGGATGTCATATTGGATCGCGTTGGGAATCGACAAAAAAACACAAATGAACTATCATATGAAACCGTTTTGCAAAGATACTTATGCAACTGAAGGCTCCTTAGgtaggttagtcttttctaggcacagaagagtgagaaaattctaaatttctgagcagtctgctttttgttttccttgagtttttacttaaagctcttaaaagaaatctaggcgtgaagttcgagccttgtaccataccacaacagcattccaaatgttacagAAGCGAAACAAACATTTACTGATAAAATCAGGTCAGCTGTTGAGGAAATTATCTGGAAGTCCGCTGAATATTGTGATATTCTTAGCGAGAAGTGGACAGGAATTCATGTGTCGGCCGACCAACTGAAAGGTCAAAGAAATAAGCAAGAAGATCGTTTTGTGGCTTATCCAAATGGACAATACATGAATCGTGGACAGgttagtgcgaatcggggactcaagatttactgaaatagtgaagagaaaacaaaagaaaactatattttcaaaaaaaatgagaactctaataaacagaatgaaaaacattcaaagctacagtagtatttccagctggagtttccagagccaaaaaaatgcgagtattactgtagttttgaaattggtttctcactttacgtacgattttttgatttttttttcagactcttcatatgaaaaaaaatcatgttttctcctttacaagatttttttgatctcaaaacatttccagAGTGACATTTCACTTCTTGCGGTGTTCGATGGGCATGGCGGACACGAGTGCTCTCAATATGCAGCTGCTCATTTCTGGGAAGCATGGTCCGATGCTCAACATCATCATTCACAAGATATGAAACTTGACGAACTCCTAGAAAAGGCTCTAGAAACATTGGACGAAAGAATGACAGTCAGAAGTGTTCGAGAATCTTGGAAAGGTGGAACCACTGCTGTCTGCTGTGCTGTTGATTTGAACACTAATCAAATCGCATTTGCCTGGCTTGGAGATTCACCAGGgtaatcaatttttttttagtttttggaactttacgtcccgaaaaattattcctttatcacctaattcctacagtaacccaagctccgaattaaataaagttaaagcgtggtatacacataaaaataagaaaaaattgttcatgaaatccatttttccagTTACATCATGTCAAACTTGGAGTTCCGCAAATTCACTACTGAACACTCCCCGTCTGACCCGGAGGAATGTCGACGAGTCGAAGAAGTCGGTGGCCAGATTTTTGTGATCGGTGGTGAGCTCCGTGTGAATGGAGTACTCAACCTGACGCGAGCACTAGGAGACGTACCTGGAAGACCAATGATATCCAACAAACCTGATACCTTACTGAAGACGATCGAACCTGCGGATTATCTTGTTTTGTTGGCCTGTGACGGGATTTCTGACGTCTTCAACACTAGTGATTTGTACAATTTGGTTCAGGCTTTTGTCAATGAATATGACGTAGAAGgtatcaaactgatcgtttttcacatcacaaaattcttgaattttccagATTATCACGAACTTGCACGCTACATTTGCAATCAAGCAGTTTCAGCTGGAAGTGCTGACAATGTGACAGTAGTTATAGGTTTCCTCCGTCCACCAGAAGACGTTTGGCGTGTAATGAAAACAGACTCGGATGATGAAGAGAGCGAGCTCGAGGAAGAAGATGACAATGAATAGtttattgcaagttttccaaaacttttccaatttccctgggtattgattagcatccatatcttacggcgattatatcaattgtaacattatttctgtttctccccccacctctcaaattttcaaatgaccctttttcttttcgtctacctgtatcgttttccattcatctccccccctccactgtggtatatcattttgtcattagaaagtattattttgattttcattggcagtagaagacaacaggatacagaagaggttttcacag 将特征序列模式以5'端至3'端方向输入以下区域。一个例子：/ac[gt]agcct/ (My pattern's name)。两个双斜线表示特征序列边界，园括号内为特征序列名称，方括号内为退化位点可能的碱基。可以输入多个特征序列，并以逗号分隔。引物显示工具自动构建每个引物的反补序列并与反链配对。不正确输入特征序列可能导致错误。 /ATGTCTGATTCGCTAAATCATCC/ (PS1), /CTATTCATTGTCATCTTCTTCC/ (PS2), /AT[AC]GT[CG]ATTGGATGT[CG]ATATTGG/ (PS3), /aacagctatgaccatg/ (reverse primer), /attaaccctcactaaag/ (T3 primer), /cgaggtcgacggtatcg/ (KS primer), /tctagaactagtggatc/ (SK primer), /aatacgactcactatag/ (T7 primer), /gtaaaacgacggccagt/ (M13 primer), /gttttcccagtcacgac/ (-40 primer)
输入遗传密码表达式。缺省的表达式为标准遗传密码表达式。斜线标出对应的特征序列的边界，等号与字母确定特征序列代表的氨基酸。方括号表示在一个位置可能的碱基，竖线分开的两个特征序列代表了同样的氨基酸。除了最后一个表达式，每个表达式后有一个逗号。 /gc[agtcn]/=A, /tg[ct]/=C, /ga[tc]/=D, /ga[ag]/=E, /tt[tc]/=F, /gg[agctn]/=G, /ca[tc]/=H, /at[atc]/=I, /aa[ag]/=K, /ct[agtcn]|tt[ag]/=L, /atg/=M, /aa[tc]/=N, /cc[atgcn]/=P, /ca[ag]/=Q, /cg[agctn]|ag[ag]/=R, /tc[agctn]|ag[ct]/=S, /ac[agctn]/=T, /gt[agctn]/=V, /tgg/=W, /ta[tc]/=Y, /ta[ga]|tga/=*